Research Paper Volume 13, Issue 7 pp 9838—9858

lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis

SNHG4 is correlated with cyclin-dependent kinase 1 (CDK1) and the CRC cell cycle. (A) Samples in GSE106582 and GSE74602 were divided into high SNHG4 expression and low SNHG4 expression groups. The correlation between SNHG4 and differentially expressed genes was analyzed to identify genes that are significantly positively correlated with SNHG4 (r > 0.40, p B–D) Correlation of the SNHG4 and CDK1 expression levels based on data from GSE8671, TCGA, and GTEx. (E) The protein levels of CDK1 in tissue samples as determined by immunoblotting. (F) The expression of CDK1 in tissue samples as determined by real-time PCR. (G) The correlation between the SNHG4 and CDK1 expression levels as determined by Pearson’s correlation analysis. (H) HCT116 and SW620 cells were transfected with si-SNHG4, and the cell cycle was examined by flow cytometry. (I) HCT116 and SW620 cells were transfected with si-SNHG4, and the protein levels of CDK1, cyclin B1, and cyclin A2 were examined. (J) HCT116 and SW620 cells were transfected with si-SNHG4 and/or CDK1 overexpression vector, and cell proliferation was examined by CCK-8 assay. (K) HCT116 and SW620 cells were transfected with si-SNHG4 and/or CDK1 overexpression vector, and cell cycle progression was examined by flow cytometry. *P **P ##P

Figure 3. SNHG4 is correlated with cyclin-dependent kinase 1 (CDK1) and the CRC cell cycle. (A) Samples in GSE106582 and GSE74602 were divided into high SNHG4 expression and low SNHG4 expression groups. The correlation between SNHG4 and differentially expressed genes was analyzed to identify genes that are significantly positively correlated with SNHG4 (r > 0.40, p < 0.05); a total of 44 genes were found to be positively correlated with SNHG4 (SKA3, TOP1MT, RFC3, DSCC1, PPIL1, UBE2C, MND1, IPO4, C10orf2, CDK1, AUNIP, DDIAS, FEN1, HELLS, CDC45, PRR7, SPC25, GGCT, KIF14, RPL13, PRMT3, PROX1, CKAP2L, SLC39A10, HMMR, KIF4A, NOLC1, EXO1, MCM6, CCNF, SPDL1, NOP16, KIF20A, MCM10, EPHX4, MMP10, FJX1, NUF2, CBX2, NEK2, ANGPT2, ECT2, KIF23, and ESM1). (B–D) Correlation of the SNHG4 and CDK1 expression levels based on data from GSE8671, TCGA, and GTEx. (E) The protein levels of CDK1 in tissue samples as determined by immunoblotting. (F) The expression of CDK1 in tissue samples as determined by real-time PCR. (G) The correlation between the SNHG4 and CDK1 expression levels as determined by Pearson’s correlation analysis. (H) HCT116 and SW620 cells were transfected with si-SNHG4, and the cell cycle was examined by flow cytometry. (I) HCT116 and SW620 cells were transfected with si-SNHG4, and the protein levels of CDK1, cyclin B1, and cyclin A2 were examined. (J) HCT116 and SW620 cells were transfected with si-SNHG4 and/or CDK1 overexpression vector, and cell proliferation was examined by CCK-8 assay. (K) HCT116 and SW620 cells were transfected with si-SNHG4 and/or CDK1 overexpression vector, and cell cycle progression was examined by flow cytometry. *P < 0.05, **P < 0.01, ##P < 0.01.