Research Paper Volume 13, Issue 7 pp 9874—9899

DNASE1L3 arrests tumor angiogenesis by impairing the senescence-associated secretory phenotype in response to stress

Downregulated DNASE1L3 is positively correlated with prognosis of resectable or unresectable HCC. (A) The transcriptional level of DNASE1L3 was down-regulated in HCC tissues(n=125) compared to paired para-tumor tissues as tested by RT-qPCR. (B) Representative images of IHC staining for DNASE1L3 in HCC and adjacent normal tissues (scale bar, 50 μm). (C) The histological scores of DNASE1L3 in 204 paired tissues of HCC was evaluated. (D) The translational level of DNASE1L3 between HCC tissues and paired adjacent non-tumor tissues from 21 patients were identified by western blotting. (“T” for tumor, “N” for non-tumor). (E, F) Kaplan-Meier survival curves of OS and RFS time for 204 patients with HCC. (G) Forest plot of risk factors of the OS time using multivariate Cox regression analysis. (H) Comparison of plasma levels of DNASE1L3 between patients of HCC(n=50), patients of hepatitis only(n=27) and healthy individuals(n=18). (I, J) Kaplan-Meier survival curves of OS and PFS time for 50 patients with inoperable HCC. (K) RT-qPCR analysis of DNASE1L3 in HCC cell lines and a normal liver cell line (THLE-3). (L) Western blot analysis of DNASE1L3 protein expression in HCC cell lines and a normal liver cell line (THLE-3).

Figure 1. Downregulated DNASE1L3 is positively correlated with prognosis of resectable or unresectable HCC. (A) The transcriptional level of DNASE1L3 was down-regulated in HCC tissues(n=125) compared to paired para-tumor tissues as tested by RT-qPCR. (B) Representative images of IHC staining for DNASE1L3 in HCC and adjacent normal tissues (scale bar, 50 μm). (C) The histological scores of DNASE1L3 in 204 paired tissues of HCC was evaluated. (D) The translational level of DNASE1L3 between HCC tissues and paired adjacent non-tumor tissues from 21 patients were identified by western blotting. (“T” for tumor, “N” for non-tumor). (E, F) Kaplan-Meier survival curves of OS and RFS time for 204 patients with HCC. (G) Forest plot of risk factors of the OS time using multivariate Cox regression analysis. (H) Comparison of plasma levels of DNASE1L3 between patients of HCC(n=50), patients of hepatitis only(n=27) and healthy individuals(n=18). (I, J) Kaplan-Meier survival curves of OS and PFS time for 50 patients with inoperable HCC. (K) RT-qPCR analysis of DNASE1L3 in HCC cell lines and a normal liver cell line (THLE-3). (L) Western blot analysis of DNASE1L3 protein expression in HCC cell lines and a normal liver cell line (THLE-3).