Figure 2. Overexpression of miR-512-5p inhibits GBM cell proliferation and causes cell cycle arrest. (A) miR-512-5p expression in U87 and LN229 cells determined after transfecting cells with corresponding vectors by RT-qPCR. (B) Cell proliferation activity as examined by EdU assays. (200 ×) (C) Cell proliferation activity as examined by colony formation assays. (D) Analysis of cell cycle by flow cytometry. (E) G1-arrest-relevant cell cycle regulators determined by western blotting. Data are represented as the mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01, Student's t-test, compared to the Control group.