Research Paper Volume 13, Issue 7 pp 9911—9926

Exosomes derived from microRNA-512-5p-transfected bone mesenchymal stem cells inhibit glioblastoma progression by targeting JAG1

BMSC-exosomal miR-512-5p can be internalized by U87 cells. (A) Surface marker proteins of BMSC identified by flow cytometry assays. (B) Representative TEM image of BMSC exosomes. (scale bar, 200 μM) (C) Particle size distribution of BMSC exosomes as measured by DLS. (D) Exosome surface markers determined by western blotting. (E) miR-512-5p expression found in BMSC and BMSC-derived exosomes by RT-qPCR after transfection with miR-512-5p or the Control. (F) BMSC-exosomal miR-512-5p internalized by U87 cells. (scar bar = 25 μM). (G) miR-512-5p and JAG1 expression determined by RT-qPCR in U87 cells after co-culture with BMSC-derived exosomes or PBS. (H) JAG1 expression determined by western blotting in U87 cells after co-culture with BMSC-derived exosomes or PBS. Data are represented as the mean ± standard deviation of three independent experiments. *p **p t-test, compared to the Control group.

Figure 5. BMSC-exosomal miR-512-5p can be internalized by U87 cells. (A) Surface marker proteins of BMSC identified by flow cytometry assays. (B) Representative TEM image of BMSC exosomes. (scale bar, 200 μM) (C) Particle size distribution of BMSC exosomes as measured by DLS. (D) Exosome surface markers determined by western blotting. (E) miR-512-5p expression found in BMSC and BMSC-derived exosomes by RT-qPCR after transfection with miR-512-5p or the Control. (F) BMSC-exosomal miR-512-5p internalized by U87 cells. (scar bar = 25 μM). (G) miR-512-5p and JAG1 expression determined by RT-qPCR in U87 cells after co-culture with BMSC-derived exosomes or PBS. (H) JAG1 expression determined by western blotting in U87 cells after co-culture with BMSC-derived exosomes or PBS. Data are represented as the mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01, Student's t-test, compared to the Control group.