Research Paper Volume 13, Issue 6 pp 9056—9070

Transplantation of olfactory ensheathing cells promotes the therapeutic effect of neural stem cells on spinal cord injury by inhibiting necrioptosis

Identification of OECs and NSCs: (A–H): Phenotypic characterization of OECs: The appearance of OECs observed by phase-contrast microscopy (a: scale bar: 100 μm, e: scale bar: 50 μm). Immunofluorescent staining showed P75-positive cells (B–D): scale bar: 100 μm) and S100-positive cells (F–H): scale bar: 100 μm), which demonstrated that these cells were OECs. (I–P): Phenotypic characterization of NSCs: The appearance of NSCs observed by phase-contrast microscopy (i: scale bar: 100 μm, m: scale bar: 50 μm). Immunofluorescent staining of NSCs using nestin antibody (F–H): scale bar: 100 μm). The neurospheres were positive for nestin, suggesting that they were NSCs. Single-cell immunofluorescent staining of NSCs expressing nestin (N–H): scale bar: 100 μm). The purity of NSC cells was evaluated by the percentages of nestin-positive cells and DAPI-positive cells.

Figure 1. Identification of OECs and NSCs: (AH): Phenotypic characterization of OECs: The appearance of OECs observed by phase-contrast microscopy (a: scale bar: 100 μm, e: scale bar: 50 μm). Immunofluorescent staining showed P75-positive cells (BD): scale bar: 100 μm) and S100-positive cells (FH): scale bar: 100 μm), which demonstrated that these cells were OECs. (IP): Phenotypic characterization of NSCs: The appearance of NSCs observed by phase-contrast microscopy (i: scale bar: 100 μm, m: scale bar: 50 μm). Immunofluorescent staining of NSCs using nestin antibody (FH): scale bar: 100 μm). The neurospheres were positive for nestin, suggesting that they were NSCs. Single-cell immunofluorescent staining of NSCs expressing nestin (NH): scale bar: 100 μm). The purity of NSC cells was evaluated by the percentages of nestin-positive cells and DAPI-positive cells.