Research Paper Volume 13, Issue 6 pp 9056—9070

Transplantation of olfactory ensheathing cells promotes the therapeutic effect of neural stem cells on spinal cord injury by inhibiting necrioptosis

OECs promote NSC survival in the SCI microenvironment. (A) In vitro, the morphologies of the cells were observed under an inverted microscope. Scale bar: 100 μm; (B) The proliferation rates of NSCs assessed via MTT assays (mean ± SEM; two-way ANOVA, error bar: 95% CI). (C) Effects of OECs on NSC proliferation under SCI conditions observed under an inverted microscope. Scale bar: 100 μm; (D) The proliferation rates of NSCs assessed via MTT assays (mean ± SEM; two-way ANOVA, error bar: 95% CI). (E) In vivo, the survival of NSCs in different groups: (A–C) NSC transplantation alone, where RFP-positive cells were NSCs. Scale bar: 100 μm. (D–F) Transplantation of NSCs and OECs. Scale bar: 100 μm. (F) Quantification of the survival of RFP+ cells at two weeks after transplantation (means ± SEM, n=6; P

Figure 2. OECs promote NSC survival in the SCI microenvironment. (A) In vitro, the morphologies of the cells were observed under an inverted microscope. Scale bar: 100 μm; (B) The proliferation rates of NSCs assessed via MTT assays (mean ± SEM; two-way ANOVA, error bar: 95% CI). (C) Effects of OECs on NSC proliferation under SCI conditions observed under an inverted microscope. Scale bar: 100 μm; (D) The proliferation rates of NSCs assessed via MTT assays (mean ± SEM; two-way ANOVA, error bar: 95% CI). (E) In vivo, the survival of NSCs in different groups: (AC) NSC transplantation alone, where RFP-positive cells were NSCs. Scale bar: 100 μm. (DF) Transplantation of NSCs and OECs. Scale bar: 100 μm. (F) Quantification of the survival of RFP+ cells at two weeks after transplantation (means ± SEM, n=6; P<0.05, one-way ANOVA).