Research Paper Volume 13, Issue 6 pp 9056—9070

Transplantation of olfactory ensheathing cells promotes the therapeutic effect of neural stem cells on spinal cord injury by inhibiting necrioptosis

Western-blot analysis reveals that OECs downregulate the expression of RIP3 and MLKL and inhibit SCI-induced increases of RIP3 and MLKL. (A) In-vitro Western-blotting analysis showed that SCI induced upregulated expression of RIP3 and MLKL, and that OECs counteracted these changes to protect NSCs. (a) The protein expression level evaluated via Western blotting. (b–d) Data presented are expressed as the mean ± SEM (n = 6; one-way ANOVA, post-hoc test: Dunn-Bonferroni; error bar: 95% CI; #P>0.05 and except for # in the figure; P values were all less than 0.05). (B) Two weeks after transplantation, protein levels of NF200, RIP3, and MLKL showed that OECs suppressed RIP3/MLKL-mediated necroptosis and protected NSCs. (a) The expression levels of NF200, RIP3, and MLKL were detected by Western blotting. (b–d) Data presented are expressed as the mean ± SEM (n = 6; one-way ANOVA, post-hoc test: Dunn-Bonferroni; error bar: 95% CI; #P>0.05, except for the # in the figure; P values were all less than 0.05).

Figure 3. Western-blot analysis reveals that OECs downregulate the expression of RIP3 and MLKL and inhibit SCI-induced increases of RIP3 and MLKL. (A) In-vitro Western-blotting analysis showed that SCI induced upregulated expression of RIP3 and MLKL, and that OECs counteracted these changes to protect NSCs. (a) The protein expression level evaluated via Western blotting. (bd) Data presented are expressed as the mean ± SEM (n = 6; one-way ANOVA, post-hoc test: Dunn-Bonferroni; error bar: 95% CI; #P>0.05 and except for # in the figure; P values were all less than 0.05). (B) Two weeks after transplantation, protein levels of NF200, RIP3, and MLKL showed that OECs suppressed RIP3/MLKL-mediated necroptosis and protected NSCs. (a) The expression levels of NF200, RIP3, and MLKL were detected by Western blotting. (bd) Data presented are expressed as the mean ± SEM (n = 6; one-way ANOVA, post-hoc test: Dunn-Bonferroni; error bar: 95% CI; #P>0.05, except for the # in the figure; P values were all less than 0.05).