Research Paper Volume 13, Issue 7 pp 10034—10057

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients

Expression levels of the five prognostic proteins in LADC patient samples. (A) Western blot analysis shows expression levels of IGF2BP1, IGF2BP2, HNRNPA2B1, HNRNPC, and METTL3 proteins in 10 paired normal lung and LADC tissue samples. (B–F) Relative expression levels of the five potentially prognostic m6A regulatory proteins, namely, IGF2BP1, IGF2BP2, HNRNPA2B1, HNRNPC, and METTL3 in 10 LADC tissues. GAPDH was used as loading control. The values were normalized by log2 fold change (ratio of tumor to normal tissue expression) of the target proteins.

Figure 4. Expression levels of the five prognostic proteins in LADC patient samples. (A) Western blot analysis shows expression levels of IGF2BP1, IGF2BP2, HNRNPA2B1, HNRNPC, and METTL3 proteins in 10 paired normal lung and LADC tissue samples. (BF) Relative expression levels of the five potentially prognostic m6A regulatory proteins, namely, IGF2BP1, IGF2BP2, HNRNPA2B1, HNRNPC, and METTL3 in 10 LADC tissues. GAPDH was used as loading control. The values were normalized by log2 fold change (ratio of tumor to normal tissue expression) of the target proteins.