Research Paper Volume 13, Issue 6 pp 9071—9084

Long non-coding RNA THRIL inhibits miRNA-24-3p to upregulate neuropilin-1 to aggravate cerebral ischemia-reperfusion injury through regulating the nuclear factor κB p65 signaling

LncRNA THRIL targets and regulates the expression of miR-24-3p. (A) The binding site was predicted. (B) The dual luciferase reporter assay was performed to verify the combination of THRIL and miR-24-3p. (C) RIP experiment was performed to prove the combination of THRIL and miR-24-3p. (D) RT-qPCR detected THRIL overexpression efficiency. (E) RT-qPCR detected the expression of miR-24-3p in SH-SY5Y cells with THRIL knockdown or overexpression. (F) RT-qPCR detected the expression of miR-24-3p in OGD/R models. (G) RT-qPCR detected mRNA levels of miR-24-3p in MCAO. Data are shown as mean ± SD for three-independent experiments. **P

Figure 4. LncRNA THRIL targets and regulates the expression of miR-24-3p. (A) The binding site was predicted. (B) The dual luciferase reporter assay was performed to verify the combination of THRIL and miR-24-3p. (C) RIP experiment was performed to prove the combination of THRIL and miR-24-3p. (D) RT-qPCR detected THRIL overexpression efficiency. (E) RT-qPCR detected the expression of miR-24-3p in SH-SY5Y cells with THRIL knockdown or overexpression. (F) RT-qPCR detected the expression of miR-24-3p in OGD/R models. (G) RT-qPCR detected mRNA levels of miR-24-3p in MCAO. Data are shown as mean ± SD for three-independent experiments. **P < 0.01.