Research Paper Volume 13, Issue 6 pp 9071—9084

Long non-coding RNA THRIL inhibits miRNA-24-3p to upregulate neuropilin-1 to aggravate cerebral ischemia-reperfusion injury through regulating the nuclear factor κB p65 signaling

NRP1 was the target of miR-24-3p. (A) The binding site of NRP1 and miR-24-3p. (B) RT-qPCR was used for detection of miR-24-3p transfection efficiency. (C) The fluorescence intensity of NRP1 3'-UTR was detected by dual luciferase reporter assay. (D) Western blot detected the expression of NRP1. (E) The expression of NRP1 was significantly higher in MCAO model. (F) The expression of NRP1 was significantly higher in OGD/R model. Data are shown as mean ± SD for three-independent experiments. **P

Figure 5. NRP1 was the target of miR-24-3p. (A) The binding site of NRP1 and miR-24-3p. (B) RT-qPCR was used for detection of miR-24-3p transfection efficiency. (C) The fluorescence intensity of NRP1 3'-UTR was detected by dual luciferase reporter assay. (D) Western blot detected the expression of NRP1. (E) The expression of NRP1 was significantly higher in MCAO model. (F) The expression of NRP1 was significantly higher in OGD/R model. Data are shown as mean ± SD for three-independent experiments. **P < 0.01.