Figure 4. Orthogonal partial least squares discriminant analysis (OPLS-DA) was used to screen differential metabolites, and boxplot was used to display the results of differential metabolites. (A) The OPLS-DA of LD3 and LAD3 were compared. (B) The metabolites of LD3 and LAD3 were Levoglucosan, (s) - Mandelic acid, N-Acetyl-D-galactosamine, Glycolic acid, D-glyceric acid, Ethanolamine, Guaninosuccinic acid 3, N-Acetyl-beta-D-mannosamine 3. (C) The metabolites comparing the specific difference between LD3 and LAD3 are Benzoic acid and 4-Hydroxybenzoic acid.