Research Paper Volume 13, Issue 8 pp 11135—11149

WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA

WTAP knockdown inhibits H/R injury in AC16 cells by suppressing ATF4. (A, B) Cell apoptosis and (C, D) expression of PARP, cleaved Caspase-3, ATF4 and CHOP in AC16 cells transduced with WTAP shRNA and ATF4-overexpressing plasmid. All experiments were repeated at least three times, and data are represented as mean ± SD. ***P ###P ΔΔΔP

Figure 3. WTAP knockdown inhibits H/R injury in AC16 cells by suppressing ATF4. (A, B) Cell apoptosis and (C, D) expression of PARP, cleaved Caspase-3, ATF4 and CHOP in AC16 cells transduced with WTAP shRNA and ATF4-overexpressing plasmid. All experiments were repeated at least three times, and data are represented as mean ± SD. ***P < 0.0001 compared with control (untreated). ###P < 0.001 vs H/R. ΔΔΔP < 0.001 vs H/R+shWTAP#1.