Research Paper Volume 13, Issue 7 pp 10158—10174

Hypoxia-induced miR-27 and miR-195 regulate ATP consumption, viability, and metabolism of rat cardiomyocytes by targeting PPARγ and FASN expression

Effects of hypoxia on the mRNA levels of HIF-1α, mCPT-I, and VEGF. The isolated cardiomyocytes were maintained under normoxia and subjected to hypoxia for 48 h. The mRNA levels of (A) HIF-1α, (B) mCPT-I, and (C) VEGF were measured using RT-qPCR analysis (n = 3). *P

Figure 1. Effects of hypoxia on the mRNA levels of HIF-1α, mCPT-I, and VEGF. The isolated cardiomyocytes were maintained under normoxia and subjected to hypoxia for 48 h. The mRNA levels of (A) HIF-1α, (B) mCPT-I, and (C) VEGF were measured using RT-qPCR analysis (n = 3). *P < 0.05 and **P < 0.01 vs. the normoxia group.