Research Paper Volume 13, Issue 7 pp 10175—10186

Regulation of PINX1 expression ameliorates lipopolysaccharide-induced lung injury and alleviates cell senescence during the convalescent phase through affecting the telomerase activity

TERT overexpression or PINX1 silencing exacerbated inflammatory response at D3 while promoted the recovery of inflammatory injury on D14, whereas PINX1 overexpression presented the opposite effects in LPS-induced lung injury rats. (A) A bicinchoninic acid (BCA) Protein Assay Kit was used to test the protein concentration in BALF. (B) The contents of cells in BALF were counted by an automatic cell counter. The levels of (C) TNF-α, (D) IL-1β, (E) IL-6, and (F) IL-18 were assessed using ELISA kits. **P***P#P##P###PΔPΔΔPΔΔΔP

Figure 3. TERT overexpression or PINX1 silencing exacerbated inflammatory response at D3 while promoted the recovery of inflammatory injury on D14, whereas PINX1 overexpression presented the opposite effects in LPS-induced lung injury rats. (A) A bicinchoninic acid (BCA) Protein Assay Kit was used to test the protein concentration in BALF. (B) The contents of cells in BALF were counted by an automatic cell counter. The levels of (C) TNF-α, (D) IL-1β, (E) IL-6, and (F) IL-18 were assessed using ELISA kits. **P<0.01, ***P<0.001 vs. Control; #P<0.05, ##P<0.01, ###P<0.001 vs. Model+vector; ΔP<0.05, ΔΔP<0.01, ΔΔΔP<0.001 vs. Model+OE-PINX1.