Figure 4. The cholinergic and GABAergic neurons were increased by up-regulated the LHX8 in the HSHF diet offspring when they getting older. (A) The mRNA sequence of whole brain tissues; (B, C) the cholinergic and GABAergic cells marked protein of ACEh, Amp, CHRNA1, CHRNB1 and GAD65; the synaptic functional markers of Dynamin 1 and PSD95, and the AD biomarkers of Tau, p-Tau, APOE, CD33 and TREM2 (D). The KEGG analysis results see in the Supplementary Figure 6. Data are presented as the means ± SD of more than 3 independent experiments in Western bolting. *p <0.05 and **p <0.01 vs. the model group by one-way ANOVA, followed by one-way analysis of variance (ANOVA) at p < 0.05.