Research Paper Volume 13, Issue 6 pp 9085—9107

Nidogen-1 expression is associated with overall survival and temozolomide sensitivity in low-grade glioma patients

NID1 silencing in U87-MG and U251 glioma cells enhanced apoptosis and sensitivity to TMZ. (A) QRT-PCR analysis shows NID1 mRNA levels in si-NID1- and si-NC-transfected U251 and U87-MG glioma cell lines. (B) Representative western blot shows NID1 protein levels in si-NID1- and si-NC-transfected U251 and U87-MG glioma cell lines. (C) Representative FACS plots and histograms show percentage apoptosis in si-NID1- and si-NC-transfected U251 and U87-MG glioma cells based on AnnexinV-FITC/PI staining. (D) Representative FACS plots and histograms show percentage apoptosis in si-NID1- and si-NC-transfected U251 and U87-MG glioma cells treated with or without TMZ. As shown, NID1 silencing improved temozolomide (TMZ) sensitivity in glioma cells. Note: * PP

Figure 4. NID1 silencing in U87-MG and U251 glioma cells enhanced apoptosis and sensitivity to TMZ. (A) QRT-PCR analysis shows NID1 mRNA levels in si-NID1- and si-NC-transfected U251 and U87-MG glioma cell lines. (B) Representative western blot shows NID1 protein levels in si-NID1- and si-NC-transfected U251 and U87-MG glioma cell lines. (C) Representative FACS plots and histograms show percentage apoptosis in si-NID1- and si-NC-transfected U251 and U87-MG glioma cells based on AnnexinV-FITC/PI staining. (D) Representative FACS plots and histograms show percentage apoptosis in si-NID1- and si-NC-transfected U251 and U87-MG glioma cells treated with or without TMZ. As shown, NID1 silencing improved temozolomide (TMZ) sensitivity in glioma cells. Note: * P<0.05;** P<0.01;*** P<0.001;**** P<0.0001.