Research Paper Volume 13, Issue 7 pp 10275—10288

CircRNA hsa_circ_0006215 promotes osteogenic differentiation of BMSCs and enhances osteogenesis–angiogenesis coupling by competitively binding to miR-942-5p and regulating RUNX2 and VEGF

CircRNA hsa

Figure 2. CircRNA hsa_circ_0006215 promote BMSCs osteogenesis. Overexpression (A), and expression of knocked down (B) hsa_circ_0006215 in BMSCs determined by qRT-PCR. Expression of COL1A1, RUNX2 and OCN in BMSCs after overexpression and knockdown of hsa_circ_0006215 detected by qRT-PCR (C) and western blotting (D). Osteogenic differentiation of BMSCs after overexpression and knockdown of hsa_circ_0006215 detected by ALP and alizarin red staining (E). (F) Quantitative analysis of osteogenic differentiation of BMSCs after overexpression and knockdown of hsa_circ_0006215 using ALP (F, G). All values are expressed as means ± SD of three independent experiments. *p < 0.05, **p < 0.01.