Figure 5. ANXA4 promotes tumorigenesis and EMT in a xenograft model. (A) CCK8 assay assessing the viability of HT-29 cells transfected with HA-ANXA4. The data are shown as the mean ± SEM of three independent experiments. *p < 0.05, **p<0.01. (B) HT-29 cells stably transfected with ANXA4 were subcutaneously injected into six nude mice (2×106/120 μl per mouse). After 25 days, the mice were sacrificed, and the tumours were removed. (C) Tumour growth curves of the xenograft tumours (n=6). Tumour volume was calculated at different time points (7, 12, 18, and 25 days) using the formula V=(a×b2)/2. *p < 0.05. (D) Protein expression levels of ANXA4 and the EMT-related molecules E-cadherin, Vimentin, and Snail in tumours determined by IHC.