Figure 2. Knockdown inhibits GC cell proliferation, colony formation, and stimulates apoptosis. (A) Efficiency of siGSPT1 was evaluated by qRT-PCR and western blot. (B) HCT116 and SW480 cells were transfected with siGSPT1 or scramble control, and then assessed for cell growth rate at 48 and 72 h in CKK8 assay. (C) Effect of silencing GSPT1 on cell cycle of colon cancer cells HCT116 and SW480. (D) EdU assay to detect the effect of silencing GSPT1 on DNA synthesis of colon cancer cells HCT116 and SW480. (E) Apoptosis rate in the GSPT1 knockdown group was increased. ** P < 0.01.