Research Paper Volume 13, Issue 7 pp 10354—10368

Identification of GSPT1 as prognostic biomarker and promoter of malignant colon cancer cell phenotypes via the GSK-3β/CyclinD1 pathway

Over-expression of GSPT1 promotes cell cycle progression and inhibits apoptosis in colon cancer cells. (A) Quantitative PCR and WB were used to detect the over-expression efficiency of GSPT1 in colon cancer cell line HCT116. (B) Over-expression GSPT1 promotes cell cycle of colon cancer cell line HCT116. (C) Over-expression of GSPT1 promotes the cloning ability of colon cancer cell line HCT116. (D) Over-expression of GSPT1 inhibits the apoptosis of colon cancer cell line HCT116. * P P

Figure 3. Over-expression of GSPT1 promotes cell cycle progression and inhibits apoptosis in colon cancer cells. (A) Quantitative PCR and WB were used to detect the over-expression efficiency of GSPT1 in colon cancer cell line HCT116. (B) Over-expression GSPT1 promotes cell cycle of colon cancer cell line HCT116. (C) Over-expression of GSPT1 promotes the cloning ability of colon cancer cell line HCT116. (D) Over-expression of GSPT1 inhibits the apoptosis of colon cancer cell line HCT116. * P < 0.05, ** P < 0.01.