Figure 4. MSC-AS1 increased cell growth, cell cycle progression and inflammatory mediator secretion. (A) The expression of MSC-AS1 was detected by qRT-PCR analysis. (B) The expression of Ki-67 was measured using qRT-PCR analysis. (C) The level of CKD2 was detected by qRT-PCR analysis. (D) MSC-AS1 overexpression induced cell cycle progression in HGC-27 cells. (E) MSC-AS1 overexpression increased HGC-27 cell growth, based on CCK-8 analysis. (F) MSC-AS1 overexpression enhanced IL-1β secretion. (G) MSC-AS1 overexpression promoted IL-6 secretion. (H) Elevated expression of MSC-AS1 increased the secretion of TNF-α. GAPDH was used as the internal control. *p<0.05, **p<0.01 and ***p<0.001.