Research Paper Volume 13, Issue 7 pp 10387—10395

MSC-AS1 induced cell growth and inflammatory mediators secretion through sponging miR-142-5p/DDX5 in gastric carcinoma

MSC-AS1 induced cell growth, cycle and inflammatory mediators secretion via modulating DDX5. (A) The level of DDX5 was measured by qRT-PCR analysis. (B) The expression of ki-67 was measured by qRT-PCR assay. (C) The expression of CKD2 was detected by qRT-PCR assay. (D) Inhibition expression of DDX5 decreased cell cycle in MSC-AS1-overexpressing HGC-27 cell. (E) Cell proliferation was measured by analyzed with CCK-8 assay. (F) The expression of IL-1β was measured by ELISA. (G) The expression of IL-6 was measured by ELISA. (H) The expression of IL-6 was detected by ELISA. *p

Figure 6. MSC-AS1 induced cell growth, cycle and inflammatory mediators secretion via modulating DDX5. (A) The level of DDX5 was measured by qRT-PCR analysis. (B) The expression of ki-67 was measured by qRT-PCR assay. (C) The expression of CKD2 was detected by qRT-PCR assay. (D) Inhibition expression of DDX5 decreased cell cycle in MSC-AS1-overexpressing HGC-27 cell. (E) Cell proliferation was measured by analyzed with CCK-8 assay. (F) The expression of IL-1β was measured by ELISA. (G) The expression of IL-6 was measured by ELISA. (H) The expression of IL-6 was detected by ELISA. *p<0.05, **p<0.01 and ***p<0.001.