Figure 2. miR-224 was up-regulated in CAFs and NSCLC cells. (A) CAFs were co-cultured with NSCLC cell lines (A549) for 24 h using Transwell chambers, and a miRNA array was carried out to determine the expression of miRNAs in CAFs. Top ten miRNAs, which were significantly up-regulated or down-regulated, were shown. (B, C) CAFs were co-cultured with NSCLC cell lines (A549 and H1299) for 24 h. We determined miR-224 expression in both CAFs and NSCLC cells by RT-PCR. (D) The level of miR-224 in the co-culture medium of CAFs-NSCLCs was evaluated by RT-PCR. (E) miR-224 in HUVECs co-cultured with CAFs was detected by RT-PCR. *** represents P<0.001. N=3.