Research Paper Volume 13, Issue 7 pp 10431—10449

miR-224 aggravates cancer-associated fibroblast-induced progression of non-small cell lung cancer by modulating a positive loop of the SIRT3/AMPK/mTOR/HIF-1α axis

miR-224 promoted CAF-mediated oncogenic effects in vivo. A549 and H1299 treated with CAF-CMmiR-224 were used for the xenograft tumor experiment in nude mice. (A, B) Tumor volumes within five weeks of incubation. (C, D) Tumor images and weight at the fifth week. (E) IHC was performed to detect the proliferation (marked by Ki-67) of NSCLC cells, and the rate of Ki-67 positive cells was counted. (F) IHC was performed to detect E-cadherin and Vimentin in the tumor tissues. (G) The EMT markers of E-cadherin, Vimentin, and N-cadherin in the formed tumor tissues were detected by western blot. (H) RT-PCR was implemented to detect miR-224 expression in the tumors. *** represents P

Figure 4. miR-224 promoted CAF-mediated oncogenic effects in vivo. A549 and H1299 treated with CAF-CMmiR-224 were used for the xenograft tumor experiment in nude mice. (A, B) Tumor volumes within five weeks of incubation. (C, D) Tumor images and weight at the fifth week. (E) IHC was performed to detect the proliferation (marked by Ki-67) of NSCLC cells, and the rate of Ki-67 positive cells was counted. (F) IHC was performed to detect E-cadherin and Vimentin in the tumor tissues. (G) The EMT markers of E-cadherin, Vimentin, and N-cadherin in the formed tumor tissues were detected by western blot. (H) RT-PCR was implemented to detect miR-224 expression in the tumors. *** represents P<0.001. N=5.