Figure 6. miR-224 overexpressed CAFs modulated SIRT3-AMPK-mTOR-HIF-1α axis. (A, B) Western blot was performed to detected SIRT3-AMPK-mTOR-HIF-1α expression in NSCLC cells and cancer tissues. (C) The expression of HIF-1α in the tumors was evaluated by tissue IF. (D, E) The downstream molecules of HIF-1α, including VEGFA, ANGPT1, FLT1 and TIMP1 were detected by RT-PCR. ** represents P<0.01, *** represents P<0.001. N=3.