Figure 3. NEW-Co-culture of IhOM-MSCs with SH-SY5Y neurons attenuates apoptosis and pyroptosis under OGD/R insult. (A) The apoptosis rate of SH-SY5Y neurons as evaluated by flow cytometry with Annexin V/PI staining. (B) Expression of Pro-caspase 3 and Cle-caspase 3 in SH-SY5Y neurons as measured by western blot analysis. (C) Expression of NLRP3, ASC, caspase1, caspase8 and GSDMD in SH-SY5Y neurons as measured by western blot analysis. (D) The histogram of pyroptosis-associated proteins by western blot analysis. (OM-MSCs were replaced by MSCs in the figure. All data are presented as the mean value ± SD. *p<0.05, **p<0.01).