Research Paper Volume 13, Issue 7 pp 10517—10534

Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats

BMSC-CM enhanced the proliferation and migration ability of fibroblasts in vitro. (A) Positive staining of vimentin and negative staining of α-SMA in cultured fibroblasts by immunofluorescence (magnification, ×200). Scale bar = 200 μm. (B) Wound–scratch assay in fibroblasts treated with different media at 0 h, 24 h, 48 h (magnification, ×40). Scale bar = 400μm. (C) The percentage of migration area in different groups. (D) Cell number displayed as OD value of fibroblasts treated with different media in the CCK-8 assay. Data are shown as means ± standard deviation (SD). *P **P ***P

Figure 1. BMSC-CM enhanced the proliferation and migration ability of fibroblasts in vitro. (A) Positive staining of vimentin and negative staining of α-SMA in cultured fibroblasts by immunofluorescence (magnification, ×200). Scale bar = 200 μm. (B) Wound–scratch assay in fibroblasts treated with different media at 0 h, 24 h, 48 h (magnification, ×40). Scale bar = 400μm. (C) The percentage of migration area in different groups. (D) Cell number displayed as OD value of fibroblasts treated with different media in the CCK-8 assay. Data are shown as means ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.