Research Paper Volume 13, Issue 7 pp 10584—10602

Suppression of choroidal neovascularization by silencing of long non-coding RNA IPW

LncRNA-IPW is significantly up-regulated in laser-induced CNV lesions and endothelial cells upon hypoxic stress. (A) The expression of nucleus control transcript (U6), cytoplasm control transcript (GAPDH), and IPW was detected by qRT-PCRs in the nucleus and cytoplasm fractions of RF/6A cells (n = 4). (B) qRT-PCRs were performed to detect the expression of IPW in the RPE/choroid complex of C57BL/6J mice on day 3, day 7, and day 14 after laser photocoagulation (n = 5 animal per group; *P U test). (C) RF/6A cells were exposed to 200 μM CoCl2 for the indicated time points. qRT-PCRs were performed to detect the expression of IPW (n = 4; *P t-test).

Figure 1. LncRNA-IPW is significantly up-regulated in laser-induced CNV lesions and endothelial cells upon hypoxic stress. (A) The expression of nucleus control transcript (U6), cytoplasm control transcript (GAPDH), and IPW was detected by qRT-PCRs in the nucleus and cytoplasm fractions of RF/6A cells (n = 4). (B) qRT-PCRs were performed to detect the expression of IPW in the RPE/choroid complex of C57BL/6J mice on day 3, day 7, and day 14 after laser photocoagulation (n = 5 animal per group; *P < 0.05; Mann-Whitney U test). (C) RF/6A cells were exposed to 200 μM CoCl2 for the indicated time points. qRT-PCRs were performed to detect the expression of IPW (n = 4; *P < 0.05; Student’s t-test).