Research Paper Volume 13, Issue 7 pp 10584—10602

Suppression of choroidal neovascularization by silencing of long non-coding RNA IPW

LncRNA-IPW regulates choroidal sprouting in ex vivo explant model. C57BL/6 mice received an intravitreal injection of Scr shRNA, IPW shRNA, vehicle (Vector), IPW overexpression vector (IPW OE), or left untreated (Ctrl, A-C). On day 14, RPE/choroid complexes were dissected. The peripheral regions of RPE complexes were cut into 1 mm × 1 mm pieces and seeded. The sprouting potency of choroidal explants were photographed on day 4, day 5, and day 6 (n = 6; Kruskal-Wallis test). The representative images of choroidal sprouting were shown. Meanwhile, the quantification results of sprouting area were shown. Scale bar: 1 mm.*P #P

Figure 4. LncRNA-IPW regulates choroidal sprouting in ex vivo explant model. C57BL/6 mice received an intravitreal injection of Scr shRNA, IPW shRNA, vehicle (Vector), IPW overexpression vector (IPW OE), or left untreated (Ctrl, A-C). On day 14, RPE/choroid complexes were dissected. The peripheral regions of RPE complexes were cut into 1 mm × 1 mm pieces and seeded. The sprouting potency of choroidal explants were photographed on day 4, day 5, and day 6 (n = 6; Kruskal-Wallis test). The representative images of choroidal sprouting were shown. Meanwhile, the quantification results of sprouting area were shown. Scale bar: 1 mm.*P < 0.05 IPW shRNA versus Ctrl group; #P < 0.05 IPW OE versus Ctrl group.