Figure 3. Effect of C2dat2 down-regulation on apoptosis in N2a cells after OGD/R. RT-qPCR manifested that C2dat2 knockdown abolished OGD/R-induced (A) C2dat2, (B) miR-30d-5p, and (C) DDIT4 expression in N2a cells. (D) Cell survival was examined by the CCK-8 assay after OGD/R with si-NT, si-C2dat2, or Z-VAD(OMe)-FMK (20 μM, 24 h) treatment. (E) Representative images of TUNEL staining after OGD/R with si-NT, si-C2dat2, or Z-VAD(OMe)-FMK (20 μM, 24 h) treatment. (F) TUNEL-positive cell rate in each group. *P < 0.05; **P < 0.01 vs. normoxia; #P < 0.05, ##P < 0.01 vs. si-NT; ns, not significant versus normoxia.