Figure 5. C2dat2 mediated a catalytic role in ischemic brain injury in vivo. (A–C) RT-qPCR showed the changes in (A) C2dat2, (B) miR-30d-5p, and (C) DDIT4 expression after infection with si-C2dat2 and Ad-C2dat2 in mice after 1 h MCAO and 24 h reperfusion. (D) Apoptosis in the ischemic penumbra was analyzed via TUNEL staining. Representative images showed TUNEL-positive cells in tissue from different groups. (E) Apoptotic rate in each group. (F) Representative images showing Nissl staining to determine neural cell loss in the ischemic penumbra. (G) Quantitative analysis of the effect of C2dat2 on neural cell loss. Data are mean ± SEM. *P < 0.05; **P < 0.01 vs. sham; #P < 0.05; ##P < 0.01 vs. si-NC or Ad-NC; aP < 0.05; aaP < 0.01 vs. saline (n = 3 per group).