Figure 4. The effect of TGF-β1 and COX-2 knockdown on the osteogenesis induced by BMP9 in C3H10T1/2 cells. (A) The 3D-reconstruction of μ-CT shows that bone formation was affected by TGF-β1, COX-2 knockdown, and/or BMP9. Scale bar = 2.5 mm. (upper panel: reconstructed bone samples; lower panel: heat map showing the bone density of the samples). (B) Quantification of μ-CT shows that the BMP9-induced bone formation was affected by TGF-β1 and COX-2. (C) Histological staining shows the osteogenesis ability was affected by TGF-β1 and/or COX-2. Scale bar = 100 μm. (Upper: H&E staining; lower: Masson trichrome staining).