Research Paper Volume 13, Issue 8 pp 11336—11351

COX-2 promotes the osteogenic potential of BMP9 through TGF-β1/p38 signaling in mesenchymal stem cells

The effect of BMP9 and/or TGF-β1 on the p38 signaling in MSCs. (A) Western blotting shows that the level of Smad2/3 or p-Smad2/3 was affected by TGF-β1 and/or BMP9. (B) Quantification results of western blots shows that the level of Smad2/3 or p-Smad2/3 was affected by COX-2 and/or BMP9. (C) Western blotting shows the effects of TGF-β1 and BMP9 on the levels of Smad1/5/8 and p-Smad1/5/8. (D) Quantification of western blots shows that the levels of Smad1/5/8 and p-Smad1/5/8 were affected by BMP9. (E) Western blotting shows the effect of TGF-β1 and/or BMP9 on the level of p38 and p-p38. (F) Quantification results of western blots shows the level p38 and p-p38 was affected by TGF-β1 and/or BMP9. (G) Western blotting shows that the level of p38 and p-p38 was affected by LY364947 and/or BMP9. (H) Quantification of western blots shows that the level of p38 and p-p38 was affected by LY364947 and/or BMP9. LY364947: TGF-βRI specific inhibitor. “**”p “#”p “##”p

Figure 5. The effect of BMP9 and/or TGF-β1 on the p38 signaling in MSCs. (A) Western blotting shows that the level of Smad2/3 or p-Smad2/3 was affected by TGF-β1 and/or BMP9. (B) Quantification results of western blots shows that the level of Smad2/3 or p-Smad2/3 was affected by COX-2 and/or BMP9. (C) Western blotting shows the effects of TGF-β1 and BMP9 on the levels of Smad1/5/8 and p-Smad1/5/8. (D) Quantification of western blots shows that the levels of Smad1/5/8 and p-Smad1/5/8 were affected by BMP9. (E) Western blotting shows the effect of TGF-β1 and/or BMP9 on the level of p38 and p-p38. (F) Quantification results of western blots shows the level p38 and p-p38 was affected by TGF-β1 and/or BMP9. (G) Western blotting shows that the level of p38 and p-p38 was affected by LY364947 and/or BMP9. (H) Quantification of western blots shows that the level of p38 and p-p38 was affected by LY364947 and/or BMP9. LY364947: TGF-βRI specific inhibitor. “**”p < 0.01, “#”p < 0.05, and “##”p < 0.01.