Research Paper Volume 13, Issue 8 pp 11433—11454

Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation

Microarray analysis of lncRNAs (long non-coding RNA) and mRNAs in P1 and P5 chondrocytes (P1: A1, A2, A3; P5: B1, B2, B3). (A) Thermal map of lncRNAs, y-axis: relative intensity of lncRNAs; (B) scatter plot of lncRNAs, Pearson correlation = 0.9853, upregulated (red) lncRNAs = 334, downregulated (green) lncRNAs = 381, not differentially expressed (black) lncRNAs = 19,376; (C) volcano plot of upregulated lncRNAs (red) = 49, downregulated (green) lncRNAs = 83, not differentially expressed (black) =19,959. (D) Thermal map of mRNAs, y-axis: relative intensity of mRNAs. (E) Scatter plot of mRNAs, Pearson correlation = 0.9885, upregulated (red) mRNAs = 212, downregulated (green) mRNAs = 451, not differentially expressed (black) mRNAs = 16,380. (F) volcano plot of mRNAs, upregulated (red) mRNAs = 51, downregulated (green) mRNAs = 191, not differentially expressed (black) mRNAs = 16,801. (G) Top ten downregulated biological processes, cellular components, and molecular functions in P5 chondrocytes compared to P1 cells. (H) Top ten upregulated biological processes, cellular components, and molecular functions in P5 chondrocytes. (I) Top eight downregulated pathways in P5 chondrocytes. (J) Top nine upregulated pathways in P5 chondrocytes.

Figure 2. Microarray analysis of lncRNAs (long non-coding RNA) and mRNAs in P1 and P5 chondrocytes (P1: A1, A2, A3; P5: B1, B2, B3). (A) Thermal map of lncRNAs, y-axis: relative intensity of lncRNAs; (B) scatter plot of lncRNAs, Pearson correlation = 0.9853, upregulated (red) lncRNAs = 334, downregulated (green) lncRNAs = 381, not differentially expressed (black) lncRNAs = 19,376; (C) volcano plot of upregulated lncRNAs (red) = 49, downregulated (green) lncRNAs = 83, not differentially expressed (black) =19,959. (D) Thermal map of mRNAs, y-axis: relative intensity of mRNAs. (E) Scatter plot of mRNAs, Pearson correlation = 0.9885, upregulated (red) mRNAs = 212, downregulated (green) mRNAs = 451, not differentially expressed (black) mRNAs = 16,380. (F) volcano plot of mRNAs, upregulated (red) mRNAs = 51, downregulated (green) mRNAs = 191, not differentially expressed (black) mRNAs = 16,801. (G) Top ten downregulated biological processes, cellular components, and molecular functions in P5 chondrocytes compared to P1 cells. (H) Top ten upregulated biological processes, cellular components, and molecular functions in P5 chondrocytes. (I) Top eight downregulated pathways in P5 chondrocytes. (J) Top nine upregulated pathways in P5 chondrocytes.