Figure 5. Agomelatine treated hypoxic endothelial cells inhibit macrophage migration. Brain bEnd.3 endothelial cells were incubated with 10 μM Agomelatine in the process of hypoxia/ reperfusion. (A). A representative schematic of the two-chamber two-cell trans-well assay. Brain bEnd.3 endothelial cells were subjected to hypoxia/reoxygenation (H/R) are plated in the bottom chamber and macrophages (IC21) are suspended in the top chamber and allowed to migrate. (B). Migrated IC21 macrophages were stained with DAPI; (C). Quantification of migrated macrophages. Scale bar, 100 μM (****P < 0.0001 vs. vehicle group; ####P < 0.0001 vs. H/R group).