Research Paper Volume 13, Issue 8 pp 11455—11469

Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF-κB axis

The effect of HOXA11-AS and miR-124-3p on MPTP-mediated SH-SY5Y neuronal damage. An in vitro model of PD was induced by MPTP on SH-SY5Y neuron cells. (A, B) RT-PCR was used to monitor HOXA11-AS and miR-124-3p expressions in SH-SY5Y cells. (C, D) Brdu and Western blot experiments were carried out to test cell viability and expression of apoptosis-related proteins in SH-SY5Y cells. (E, F) Western blot was applied to verify the protein expression of NLRP3 inflammasome, FSTL1 and p-NF-κB in SH-SY5Y cells. ** P P P P P P P

Figure 2. The effect of HOXA11-AS and miR-124-3p on MPTP-mediated SH-SY5Y neuronal damage. An in vitro model of PD was induced by MPTP on SH-SY5Y neuron cells. (A, B) RT-PCR was used to monitor HOXA11-AS and miR-124-3p expressions in SH-SY5Y cells. (C, D) Brdu and Western blot experiments were carried out to test cell viability and expression of apoptosis-related proteins in SH-SY5Y cells. (E, F) Western blot was applied to verify the protein expression of NLRP3 inflammasome, FSTL1 and p-NF-κB in SH-SY5Y cells. ** P <0.01, *** P <0.001 (vs.con group), & P <0.05, && P <0.01, &&& P <0.001 (vs.MPTP group), #P <0.05, ### P <0.001 (vs.MPTP + miR-124-3p group).