Research Paper Volume 13, Issue 8 pp 11455—11469

Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF-κB axis

Effect of HOXA11-AS and miR-124-3p on LPS-mediated activation of BV2. An in vitro model of PD was induced by LPS on BV2 cells. (A, B) RT-PCR was employed to monitor HOXA11-AS and miR-124-3p expressions in BV2 microglia cells. (C) RT-PCR was carried out to test the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in BV2 microglia cells. (D, E) Western blot test was performed to determine the protein expression of NLRP3 inflammasome, FSTL1 and p-NF-κB in BV2 microglia cells. ** P P P P

Figure 3. Effect of HOXA11-AS and miR-124-3p on LPS-mediated activation of BV2. An in vitro model of PD was induced by LPS on BV2 cells. (A, B) RT-PCR was employed to monitor HOXA11-AS and miR-124-3p expressions in BV2 microglia cells. (C) RT-PCR was carried out to test the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in BV2 microglia cells. (D, E) Western blot test was performed to determine the protein expression of NLRP3 inflammasome, FSTL1 and p-NF-κB in BV2 microglia cells. ** P <0.01, *** P <0.001 (vs.con group), && P <0.01, &&& P <0.001 (vs.LPS group), ### P <0.001 (vs. LPS + miR-124-3p group).