Research Paper Volume 13, Issue 8 pp 11455—11469

Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF-κB axis

Inhibition of NF-κB attenuated HOXA11-AS-mediated neuronal damage and microglia activation. MPTP induced SH-SY5Y cells and LPS induced BV2 cells were transfected HOXA11-AS overexpressing and/or treated with BAY 11-7082 (1 μmol/L). (A) Brdu assay was conducted to test the cell viability of SH-SY5Y cells. (B–D) Western blot was utilized to compare the expression of apoptosis-related proteins (Bax, Caspase3 and Bcl2), NLRP3 inflammasome, FSTL1 and NF-κB in SH-SY5Y cells. (E) RT-PCR was carried out to test the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in BV2 cells. (F, G) Western blot was employed to monitor the protein expression of NLRP3 inflammasome, FSTL1 and NF-κB in BV2 cells. ** P P P P

Figure 5. Inhibition of NF-κB attenuated HOXA11-AS-mediated neuronal damage and microglia activation. MPTP induced SH-SY5Y cells and LPS induced BV2 cells were transfected HOXA11-AS overexpressing and/or treated with BAY 11-7082 (1 μmol/L). (A) Brdu assay was conducted to test the cell viability of SH-SY5Y cells. (BD) Western blot was utilized to compare the expression of apoptosis-related proteins (Bax, Caspase3 and Bcl2), NLRP3 inflammasome, FSTL1 and NF-κB in SH-SY5Y cells. (E) RT-PCR was carried out to test the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in BV2 cells. (F, G) Western blot was employed to monitor the protein expression of NLRP3 inflammasome, FSTL1 and NF-κB in BV2 cells. ** P <0.01, *** P <0.001 (vs.MPTP or LPS group), ## P <0.01, ### P <0.001 (vs.MPTP + HXOA11-AS or LPS+HOXA11-AS group).