Research Paper Volume 13, Issue 8 pp 11455—11469

Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF-κB axis

Knocking down HOXA11-AS mitigated PD progression and inflammation in mice. Si-HOXA11-AS was used to inference the level of HOXA11-AS in the brain tissues of mice, which were then subjected to MPTP to induce a PD model. (A) The neurological functions of PD mice were evaluated by spontaneous motor test, rotarod test and bevel test. (B, C) Immunohistochemistry was adopted to examine the number of TH (B) and Caspase-3 (C) positive cells in SN area. (D) RT-PCR was conducted to determine the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in SN area. (E, F) Western blot was carried out to monitor the expression of FSTL1, NF-κB and NLRP3 inflammasome in SN area. (G) The activation of microglia was tested by immunofluorescence. (H, I) RT-PCR was adopted to examine the expression of HOXA11-AS and miR-124-3p in in SN area. ns P> 0.05 (vs.PD group), ns P> 0.05, * P P P

Figure 6. Knocking down HOXA11-AS mitigated PD progression and inflammation in mice. Si-HOXA11-AS was used to inference the level of HOXA11-AS in the brain tissues of mice, which were then subjected to MPTP to induce a PD model. (A) The neurological functions of PD mice were evaluated by spontaneous motor test, rotarod test and bevel test. (B, C) Immunohistochemistry was adopted to examine the number of TH (B) and Caspase-3 (C) positive cells in SN area. (D) RT-PCR was conducted to determine the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in SN area. (E, F) Western blot was carried out to monitor the expression of FSTL1, NF-κB and NLRP3 inflammasome in SN area. (G) The activation of microglia was tested by immunofluorescence. (H, I) RT-PCR was adopted to examine the expression of HOXA11-AS and miR-124-3p in in SN area. ns P> 0.05 (vs.PD group), ns P> 0.05, * P <0.05, ** P <0.01, *** P <0.001 (vs.PD + Si-NC group).