Research Paper Volume 13, Issue 8 pp 11470—11490

Excessive expression of miR-1a by statin causes skeletal injury through targeting mitogen-activated protein kinase kinase kinase 1

Statin induces excessive expression of miR-1a in skeletal muscle cells. (A) Cultured skeletal muscle cells were treated with simvastatin (5 μM) as indicated time points. The expressions of miR-1a was assayed using quantitative real-time PCR. N = 5 per group. **P B) Cultured skeletal muscle cells were treated with simvastatin (24 hours) as indicated concentration points. The expressions of miR-1a was assayed using quantitative real-time PCR. N = 5 per group. *P **P post-hoc tests was used to determine P value in (A, B).

Figure 1. Statin induces excessive expression of miR-1a in skeletal muscle cells. (A) Cultured skeletal muscle cells were treated with simvastatin (5 μM) as indicated time points. The expressions of miR-1a was assayed using quantitative real-time PCR. N = 5 per group. **P < 0.01 vs. control group (0 h). (B) Cultured skeletal muscle cells were treated with simvastatin (24 hours) as indicated concentration points. The expressions of miR-1a was assayed using quantitative real-time PCR. N = 5 per group. *P < 0.05 or **P < 0.01 vs. control group (0 μM). A one-way ANOVA followed by Tukey post-hoc tests was used to determine P value in (A, B).