Research Paper Volume 13, Issue 8 pp 11470—11490

Excessive expression of miR-1a by statin causes skeletal injury through targeting mitogen-activated protein kinase kinase kinase 1

Downregulation of miR-1a expression inhibits the apoptosis of gastrocnemius muscle cells induced by statin in vivo. The protocol for animal experiments was shown in Figure 6A. (A–C) Expressional levels of pro-/cleaved-caspase3, 7, 9, bcl-2 and bax in gastrocnemius were assayed by western blot. (D) Cleaved-caspase3 in gastrocnemius were assayed by IHC. (E) Cell apoptosis in gastrocnemius was determined by TUNEL method. N is 10-15 in each group. **P #P##Ppost-hoc tests was used to determine P value in (A–E).

Figure 8. Downregulation of miR-1a expression inhibits the apoptosis of gastrocnemius muscle cells induced by statin in vivo. The protocol for animal experiments was shown in Figure 6A. (AC) Expressional levels of pro-/cleaved-caspase3, 7, 9, bcl-2 and bax in gastrocnemius were assayed by western blot. (D) Cleaved-caspase3 in gastrocnemius were assayed by IHC. (E) Cell apoptosis in gastrocnemius was determined by TUNEL method. N is 10-15 in each group. **P < 0.01 vs. vehicle group. #P<0.05 or ##P<0.01 vs. statin alone. A one-way ANOVA followed by Tukey post-hoc tests was used to determine P value in (AE).