Research Paper Volume 13, Issue 7 pp 10672—10687

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway

Upregulation of NET1 in HCC tissues is associated with clinical prognosis. (A) NET1 mRNA levels in 45 pairs of N and T tissues were determined by real-time PCR. Expression of NET1 was normalized to that of GAPDH. (B) NET1 protein levels in 8 pairs of N and T tissues, and in 4 pairs of N, T and P tissues were determined by Western blot; GAPDH was used as a loading control. (C) Immunohistochemistry staining of NET1 in paired N and T tissues from two patients. Scale bars, 250 μm; 100 μm. (D) H-scores of NET1 staining intensity in N (n = 210) and T (n = 210) tissues. (E and F) Kaplan–Meier analysis of overall survival (E) and disease-free survival (F) using tissue microarray (TMA) data of 210 patients. N, non-carcinoma normal tissues; T, tumor tissues; P, portal vein tumor thrombus tissues. ***P

Figure 1. Upregulation of NET1 in HCC tissues is associated with clinical prognosis. (A) NET1 mRNA levels in 45 pairs of N and T tissues were determined by real-time PCR. Expression of NET1 was normalized to that of GAPDH. (B) NET1 protein levels in 8 pairs of N and T tissues, and in 4 pairs of N, T and P tissues were determined by Western blot; GAPDH was used as a loading control. (C) Immunohistochemistry staining of NET1 in paired N and T tissues from two patients. Scale bars, 250 μm; 100 μm. (D) H-scores of NET1 staining intensity in N (n = 210) and T (n = 210) tissues. (E and F) Kaplan–Meier analysis of overall survival (E) and disease-free survival (F) using tissue microarray (TMA) data of 210 patients. N, non-carcinoma normal tissues; T, tumor tissues; P, portal vein tumor thrombus tissues. ***P < 0.001.