Figure 1. Upregulation of NET1 in HCC tissues is associated with clinical prognosis. (A) NET1 mRNA levels in 45 pairs of N and T tissues were determined by real-time PCR. Expression of NET1 was normalized to that of GAPDH. (B) NET1 protein levels in 8 pairs of N and T tissues, and in 4 pairs of N, T and P tissues were determined by Western blot; GAPDH was used as a loading control. (C) Immunohistochemistry staining of NET1 in paired N and T tissues from two patients. Scale bars, 250 μm; 100 μm. (D) H-scores of NET1 staining intensity in N (n = 210) and T (n = 210) tissues. (E and F) Kaplan–Meier analysis of overall survival (E) and disease-free survival (F) using tissue microarray (TMA) data of 210 patients. N, non-carcinoma normal tissues; T, tumor tissues; P, portal vein tumor thrombus tissues. ***P < 0.001.