Research Paper Volume 13, Issue 7 pp 10672—10687

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway

NET1 overexpression promotes HCC cell proliferation, migration and invasion in vitro. (A) Western blot analysis of NET1 expression in 5 HCC cell lines. (B) Western blot analysis of NET1 overexpression efficiency in HCC cell lines (Hep3B and Huh7). (C and D) MTT assays showing the effects of NET1 overexpression on the proliferation of Hep3B (C) and Huh7 (D) cells. (E) Crystal violet assays showing the effects of NET1 overexpression on the proliferation of Hep3B and Huh7 cells. Left panel: crystal violet assay. Right panel: OD values of crystal violet assays. (F and G) Transwell assays showing the effects of NET1 overexpression on the migration (F) and invasion (G) of Hep3B and Huh7 cells. Left panel: transwell assays. Right panel: calculation of cells that have migrated (F) or invaded (G) through the filter following eosin staining. All data are presented as mean ± SE. **P ***P

Figure 2. NET1 overexpression promotes HCC cell proliferation, migration and invasion in vitro. (A) Western blot analysis of NET1 expression in 5 HCC cell lines. (B) Western blot analysis of NET1 overexpression efficiency in HCC cell lines (Hep3B and Huh7). (C and D) MTT assays showing the effects of NET1 overexpression on the proliferation of Hep3B (C) and Huh7 (D) cells. (E) Crystal violet assays showing the effects of NET1 overexpression on the proliferation of Hep3B and Huh7 cells. Left panel: crystal violet assay. Right panel: OD values of crystal violet assays. (F and G) Transwell assays showing the effects of NET1 overexpression on the migration (F) and invasion (G) of Hep3B and Huh7 cells. Left panel: transwell assays. Right panel: calculation of cells that have migrated (F) or invaded (G) through the filter following eosin staining. All data are presented as mean ± SE. **P < 0.01, ***P < 0.001 vs vector cells.