Research Paper Volume 13, Issue 7 pp 10672—10687

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway

NET1 knockdown inhibits HCC cell proliferation, migration and invasion in vitro. (A) Western blot analysis showing the knockdown efficiency of NET1 in HCC cell lines (YY-8103 and CSQT-2). (B and C) MTT assays showing the effects of NET1 knockdown on the proliferation of YY-8103 (B) and CSQT-2 (C) cells. (D) Crystal violet assays showing the effects of NET1 knockdown on the proliferation of YY-8103 and CSQT-2 cells. Left panel: crystal violet assays. Right panel: OD values of crystal violet assays. (E and F) Transwell assays showing the effects of NET1 knockdown on the migration (E) and invasion (F) of YY-8103 and CSQT-2 cells. Left panel: transwell assays. Right panel: calculation of cells that have migrated (E) or invaded (F) through the filter following eosin staining. All data are presented as mean ± SE. **P ***P

Figure 3. NET1 knockdown inhibits HCC cell proliferation, migration and invasion in vitro. (A) Western blot analysis showing the knockdown efficiency of NET1 in HCC cell lines (YY-8103 and CSQT-2). (B and C) MTT assays showing the effects of NET1 knockdown on the proliferation of YY-8103 (B) and CSQT-2 (C) cells. (D) Crystal violet assays showing the effects of NET1 knockdown on the proliferation of YY-8103 and CSQT-2 cells. Left panel: crystal violet assays. Right panel: OD values of crystal violet assays. (E and F) Transwell assays showing the effects of NET1 knockdown on the migration (E) and invasion (F) of YY-8103 and CSQT-2 cells. Left panel: transwell assays. Right panel: calculation of cells that have migrated (E) or invaded (F) through the filter following eosin staining. All data are presented as mean ± SE. **P < 0.01, ***P < 0.001 vs SCR cells.