Research Paper Volume 13, Issue 7 pp 10672—10687

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway

class="figure-viewer-img"

Figure 3. NET1 knockdown inhibits HCC cell proliferation, migration and invasion in vitro. (A) Western blot analysis showing the knockdown efficiency of NET1 in HCC cell lines (YY-8103 and CSQT-2). (B and C) MTT assays showing the effects of NET1 knockdown on the proliferation of YY-8103 (B) and CSQT-2 (C) cells. (D) Crystal violet assays showing the effects of NET1 knockdown on the proliferation of YY-8103 and CSQT-2 cells. Left panel: crystal violet assays. Right panel: OD values of crystal violet assays. (E and F) Transwell assays showing the effects of NET1 knockdown on the migration (E) and invasion (F) of YY-8103 and CSQT-2 cells. Left panel: transwell assays. Right panel: calculation of cells that have migrated (E) or invaded (F) through the filter following eosin staining. All data are presented as mean ± SE. **P < 0.01, ***P < 0.001 vs SCR cells.