Research Paper Volume 13, Issue 7 pp 10672—10687

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway

NET1 knockdown suppresses HCC tumorigenesis, and inhibits intrahepatic and lung metastases in vivo. (A) Left panel: representative images of tumors generated by control and NET1-knocked down YY-8103 cells. Middle panel: tumor weight (g). Right panel: tumor growth (mm3). (B) Left panel: representative images showing luciferase expression in intrahepatic tumors of mice from YY-8103 control and shNET1 groups. Right panel: quantification of luciferase expression in intrahepatic tumors. (C) Survival curves of mice in YY-8103 control and shNET1 groups. (D) Representative images of mice livers from YY-8103 control and shNET1 groups with intrahepatic injection. (E) Foci number of injected (left image) and non-injected (right image) lobes of mice with intrahepatic metastasis. (F) Left image: representative images showing luciferase expression from metastasized lungs of mice from YY-8103 control and shNET1 groups. Right image: quantification of luciferase expression in metastasized lungs. All data are presented as mean ± SE. *P **P ***P

Figure 4. NET1 knockdown suppresses HCC tumorigenesis, and inhibits intrahepatic and lung metastases in vivo. (A) Left panel: representative images of tumors generated by control and NET1-knocked down YY-8103 cells. Middle panel: tumor weight (g). Right panel: tumor growth (mm3). (B) Left panel: representative images showing luciferase expression in intrahepatic tumors of mice from YY-8103 control and shNET1 groups. Right panel: quantification of luciferase expression in intrahepatic tumors. (C) Survival curves of mice in YY-8103 control and shNET1 groups. (D) Representative images of mice livers from YY-8103 control and shNET1 groups with intrahepatic injection. (E) Foci number of injected (left image) and non-injected (right image) lobes of mice with intrahepatic metastasis. (F) Left image: representative images showing luciferase expression from metastasized lungs of mice from YY-8103 control and shNET1 groups. Right image: quantification of luciferase expression in metastasized lungs. All data are presented as mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001 vs SCR cells.