Research Paper Volume 13, Issue 7 pp 10672—10687

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway

NET1 overexpression promotes HCC tumorigenesis and intrahepatic metastases in vivo. (A) Left image: representative images of tumors generated by control and NET1-overexpressed Huh7 cells. Middle image: tumor weight (g). Right panel: tumor growth (mm3). (B) Left panel: representative images showing luciferase expression in intrahepatic tumors of mice from control and NET1-overexpressed groups. Right panel: quantification of luciferase expression in intrahepatic tumors. (C) Survival curves of mice in Huh7 control and NET1-overexpressed groups. (D) Representative images of mice livers from Huh7 control and NET1-overexpressed groups with intrahepatic injection. (E) Foci number of injected (left panel) and non-injected (right photograph) lobes of mice with intrahepatic metastasis. All data are presented as mean ± SE. *P **P ***P

Figure 5. NET1 overexpression promotes HCC tumorigenesis and intrahepatic metastases in vivo. (A) Left image: representative images of tumors generated by control and NET1-overexpressed Huh7 cells. Middle image: tumor weight (g). Right panel: tumor growth (mm3). (B) Left panel: representative images showing luciferase expression in intrahepatic tumors of mice from control and NET1-overexpressed groups. Right panel: quantification of luciferase expression in intrahepatic tumors. (C) Survival curves of mice in Huh7 control and NET1-overexpressed groups. (D) Representative images of mice livers from Huh7 control and NET1-overexpressed groups with intrahepatic injection. (E) Foci number of injected (left panel) and non-injected (right photograph) lobes of mice with intrahepatic metastasis. All data are presented as mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001 vs vector cells.