Research Paper Volume 13, Issue 7 pp 10672—10687

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway

NET1 activates Akt signaling in HCC cells. (A) Hierarchical clustering generated from the expression profiles of differentially expressed mRNA levels in YY-8103 control and shNET1 groups. (B) Gene set enrichment analysis of altered signaling pathways in YY-8103 control and shNET1 groups. (C) Effects of NET1 knockdown on the expression of MMP2, Cyclin D1 and Vimentin in YY-8103 cells examined by real-time PCR. ***P D) Effects of NET1 overexpression on the expression of MMP2, Cyclin D1 and Vimentin in Huh7 cells examined by real-time PCR. ***P E) Effects of NET1 overexpression on the phosphorylation of Akt in Hep3B and Huh7 cells examined by Western blot. (F) Effects of NET1 knockdown on the phosphorylation of Akt in YY-8103 and CSQT-2 cells examined by Western blot. All data are presented as mean ± SE.

Figure 6. NET1 activates Akt signaling in HCC cells. (A) Hierarchical clustering generated from the expression profiles of differentially expressed mRNA levels in YY-8103 control and shNET1 groups. (B) Gene set enrichment analysis of altered signaling pathways in YY-8103 control and shNET1 groups. (C) Effects of NET1 knockdown on the expression of MMP2, Cyclin D1 and Vimentin in YY-8103 cells examined by real-time PCR. ***P < 0.001 vs SCR cells. (D) Effects of NET1 overexpression on the expression of MMP2, Cyclin D1 and Vimentin in Huh7 cells examined by real-time PCR. ***P < 0.001 vs vector cells. (E) Effects of NET1 overexpression on the phosphorylation of Akt in Hep3B and Huh7 cells examined by Western blot. (F) Effects of NET1 knockdown on the phosphorylation of Akt in YY-8103 and CSQT-2 cells examined by Western blot. All data are presented as mean ± SE.