Research Paper Volume 13, Issue 8 pp 11528—11541

MicroRNA-532-5p protects against cerebral ischemia-reperfusion injury by directly targeting CXCL1

MiR-532-5p overexpression significantly reduces OGD/R-induced apoptosis in SH-SY5Y cells. (A) RT-qPCR analysis shows miR-532-5p levels in control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (B) RT-qPCR analysis shows miR-532-5p levels in control and miR-532-5p agomir-transfected SH-SY5Y cells. (C) CCK-8 assay analysis shows the viability of control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (D) Flow cytometry analysis of Annexin V plus propidium iodide (PI) stained control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (E) Quantitative analysis of FACS data shows the percentage of apoptotic cells (Annexin-V+ PI+ plus Annexin-V+ PI+) in control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. **P ##P

Figure 1. MiR-532-5p overexpression significantly reduces OGD/R-induced apoptosis in SH-SY5Y cells. (A) RT-qPCR analysis shows miR-532-5p levels in control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (B) RT-qPCR analysis shows miR-532-5p levels in control and miR-532-5p agomir-transfected SH-SY5Y cells. (C) CCK-8 assay analysis shows the viability of control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (D) Flow cytometry analysis of Annexin V plus propidium iodide (PI) stained control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (E) Quantitative analysis of FACS data shows the percentage of apoptotic cells (Annexin-V+ PI+ plus Annexin-V+ PI+) in control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. **P < 0.01 compared to control; ##P < 0.01 compared to OGD/R. All experiments were performed in triplicate.