Research Paper Volume 13, Issue 8 pp 11528—11541

MicroRNA-532-5p protects against cerebral ischemia-reperfusion injury by directly targeting CXCL1

MiR-532-5p overexpression significantly reduces OGD/R-induced oxidative stress in SH-SY5Y cells. (A) FACS plots show ROS levels based on DCF-DA fluorescence intensity in control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (B) The histogram plots show the levels of DCF-DA fluorescence intensity in control and miR-532-5p agomir-transfected SH SY5Y cells treated with or without OGD/R. (C–E) The histogram plots show the concentrations of (C) LDH, (D) MDA and (E) SOD in the supernatants of control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R, as analyzed using ELISA kits. **P ##P

Figure 2. MiR-532-5p overexpression significantly reduces OGD/R-induced oxidative stress in SH-SY5Y cells. (A) FACS plots show ROS levels based on DCF-DA fluorescence intensity in control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (B) The histogram plots show the levels of DCF-DA fluorescence intensity in control and miR-532-5p agomir-transfected SH SY5Y cells treated with or without OGD/R. (CE) The histogram plots show the concentrations of (C) LDH, (D) MDA and (E) SOD in the supernatants of control and miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R, as analyzed using ELISA kits. **P < 0.01 compared to control; ##P < 0.01 compared to OGD/R. All experiments were performed in triplicates.