Research Paper Volume 13, Issue 8 pp 11528—11541

MicroRNA-532-5p protects against cerebral ischemia-reperfusion injury by directly targeting CXCL1

MiR-532-5p directly targets CXCL1. (A) A portion of the 3’UTR sequence of CXCL1 from nucleotides 593-600 represents the predicted target binding site for miR-532-5p. (B) Dual luciferase reporter assay results show the relative luciferase activity in SH-SY5Y cells co-transfected with miR-532-5p agomir and luciferase vector carrying wild-type (WT) or mutant (MT) CXCL1 3′-UTR. (C) RT-qPCR analysis shows the relative expression of CXCL1 in control and miR-532-5p agomir-transfected SH-SY5Y cells. **P

Figure 4. MiR-532-5p directly targets CXCL1. (A) A portion of the 3’UTR sequence of CXCL1 from nucleotides 593-600 represents the predicted target binding site for miR-532-5p. (B) Dual luciferase reporter assay results show the relative luciferase activity in SH-SY5Y cells co-transfected with miR-532-5p agomir and luciferase vector carrying wild-type (WT) or mutant (MT) CXCL1 3′-UTR. (C) RT-qPCR analysis shows the relative expression of CXCL1 in control and miR-532-5p agomir-transfected SH-SY5Y cells. **P < 0.01 compared to control. All experiments were performed in triplicate.